Tag: gene regulation

The efficiency of animal production using somatic cell NT is very low. The doner nucleus must establish normal expression patterns to ensure that the reconstructed embryo develops successfully. To determine the possible genetic causes of death in animal production using NT techniques, the transcription of eight genes (Xist, VEGF, Hsp70.1, FGF10, PCAF, FGFR2, PDGFRa, and […]

All deceased cloned cattle died within 48 h of birth, and all had aberrations in many of their organs. The principal abnormalities at necropsy are shown in Table 2. Seven hearts from deceased clones were abnormal, displaying compensatory cardiac hypertrophy, vascular incompetence and patent foramen ovale, cardiac hemorrhage, and necrosis. At birth, atelectasis was observed […]

Nuclear Transfer The NT procedures have been described in detail by Gong et al.. The donor nuclei were obtained from skin fibroblast cells of elite Holstein cow (age, 4 yr) and from FF cells of a female fetus (gestational age, 40 days). Approximately 27.9% (n = 283) and 37.9% (n = 294) of reconstructed embryos […]

Several mammalian species have been successfully cloned by somatic cell nuclear transfer (NT), but only a small proportion of the embryos produced using adult or fetal somatic cells develop into living young (typically between 0 and 4%). Clearly, the causes of low efficiency must be identified if the medical and agricultural potentials of cloning procedures […]

In this paper, studies were done to show that an in vivo electroporation is an effective way to study promoters in the rodent epididymis, particularly in the initial segment, which is unique in its dependence on LTFs for normal gene expression and function. Control experiments using a plasmid encoding EGFP under the control of the […]

Localization of EGFP Expression after In Vivo Electroporation in the Initial Segment To test the ability of the in vivo electroporation method to express genes in the initial segment, a plasmid-encoding EGFP under the control of the cytomegalovirus (CMV) promoter was injected intraluminally into the initial segment and electroporated. After 72 h, the initial segments […]

Animals Normal adult male Sprague-Dawley rats (Hilltop Laboratories, Philadelphia, PA) were maintained on a 12L:12D cycle with free access to food and water in the University of Virginia vivarium. All experiments complied with the regulations set forth by the Animal Welfare Act (Public Law 91-579), the Guide for the Care and Use of Laboratory Animals […]

Tissue-specific gene expression can be controlled at multiple levels by such elements as cis-acting sequences, tissue-specific expression of transcription factors, and local chromatin structure. Promoter analysis is the foundation for understanding how genes are expressed in specific tissues. However, most promoter analysis experiments are carried out in cell culture, either in cells that express the […]

As revealed by the present study, connexin gene expression in the endometrium is regulated via two distinct mechanisms: The first is through an ERa-dependent signaling pathway during preimplantation; the second, an ERa-independent pathway, occurs during embryo implantation or artificial decidualization. As shown previously, maternal progesterone suppresses expression of Cx26 and Cx43 in rat endometrium during […]

ER-Mediated Regulation of Cx26 and Cx43 Gene Expression in Rat Endometrium Induction of the gap junction Cx26 in the epithelial cells and Cx43 in the developing decidual cells of rat endometrium is dependent on the blastocyst during implantation, but it is highly regulated by ovarian steroid hormones during preimplantation. To further analyze the signaling pathways […]