Tag: aeruginosa

As expected, radiation-induced leukopenia dramatically increased the mortality of septic shock. Uncontrolled infection due to immune suppression might have been responsible for this high mortality. Interestingly, this was not reflected by greater or more prolonged endotoxemia. This suggests that, in the setting of immune suppression (in this case radiation-induced leukopenia), levels of endotoxin that would […]

In a previous study using this canine model, we demonstrated that S aureus produced a cardiovascular pattern of septic shock identical to E coli9 but in the absence of any detectable endotoxemia. This indicates that bacterial products other than endotoxin can activate a common pathway resulting in similar cardiovascular injury and mortality. The current investigation […]

Most previous studies of endotoxemia in human sepsis relied on LAL assays based on gelation that were only sensitive to 1 ng/ml or more of endotoxin. In the current experiment, P aeruginosa produced such low levels of endotoxemia that dogs dying of sepsis from this Gram-negative organism would have been LAL assay negative by the […]

The detection of endotoxemia with the LAL assay in human infection has in some studies correlated poorly with clinical events and outcomes. These investigations included patients from diverse clinical settings who were infected with a wide variety of Gram-negative bacteria that differed in virulence factors, growth characteristics, and endotoxin structure. In this study, using a […]

Pseudomonas aeruginosa-infected dogs had markedly lower endotoxin levels compared with the E coli groups (p<0.0001). Eleven of 32 animals died in the P aeruginosa group compared with none of the nonirradiated E coli dogs (7 and 14 x 109 CFU/kg), despite circulating endotoxin concentrations that were more than tenfold lower in the P aeruginosa-infected dogs […]

Overall mortality differed markedly among the study groups (Table 1). All dogs receiving E coli at doses of 7 and 14 x 109 CFU/kg survived. Pseudomonas aeruginosa at a dose that was nonlethal for E coli (14 X 109 CFU/kg) resulted in a significant mortality, 11 of 32 animals (p<0.01; Fisher exact test). Total body […]

Comparison of Qualitative Hemodynamic Changes and Mortality Serial changes in cardiovascular performance were observed during the course of the experiment in all infected dogs (Fig 2 and 3, data not shown for E coli 7 X 10P CFU/kg). Animals implanted with sterile clots, however, maintained their hemodynamic parameters within the normal range (Fig 2 and […]

Clinical Manifestations, Blood Cultures, and Laboratory Tests As previously reported, the intraperitoneal implantation of infected clot produced fever and prostration in all animals.- Blood cultures grew the organism placed in the clot on days 1, 2, and 3 after surgery. Thereafter, blood culture positivity began to decrease. In survivors, blood cultures were sterile by seven […]

These data were collected to determine that early peaks or surges in endotoxemia were not missed by the chosen time points. The endotoxin contents of the E coli and P aeruginosa strains used in this study were determined on freshly grown and washed bacteria using serial dilutions. buy ampicillin Statistics The patterns of hemodynamic response […]

The concentrates and a purified recombinant exotoxin A standard (gift of Dr. David J. FitzGerald, Laboratory of Molecular Biology, National Cancer Institute) were electrophoresed on a 10 percent polyacrylamide gel followed by overnight electrophoretic blotting (Bio-Rad, Rockville Centre, NY) onto nitrocellulose membranes (Schleicher and Schuell, Inc, Keene, NH) using a pH of 8.3, 250 mmol/L […]