Pseudomonas aeruginosa Compared with Escherichia coli Produces Less Endotoxemia: Methods (2)

This resulted in bacteremia for three to four days. Cardiovascular evaluations were not obtained until at least 24 hours after surgery to allow lull recovery from anesthesia. buy levaquin online
Ten dogs were exposed to a nonlethal dose of cobalt 60 radiation, which was known to result in transient leukopenia five to ten days later, to determine the effect of leukopenia on endotoxemia during septic shock. Unanesthetized animals were secured in plexiglass holders and bilaterally exposed to a midline tissue dose of 200 centiGrays (cGy) of cobalt 60 radiation at 10 cGy per minute. Depth-dose measurements were made at the center of a cylindrical phantom (diameter of 15.2 cm, composed of 0.32-cm lucite filled with muscle-equivalent liquid). Actual irradiations were monitored with ionization chambers. Six days after radiation injury at the time of maximal leukopenia, animals were implanted with E coli-infected clots and studied as described above.

Bacteria and Fibrin Clot Preparation
The strain of E coli 06: H8 used in these studies was isolated from a patient with urinary tract sepsis. The P aeruginosa was obtained from a patient with a fatal case of septic shock. Bacteria were stored in 1-ml aliquots of bactopeptone broth (Difco, Detroit, MI) and glycerol at — 70°C. Bacterial doses and fibrin clots were prepared as previously described. The doses of bacteria were quantified turbidimetrically using a standard curve based on actual viability counts. Bacteria for direct LAL testing were prepared in the same manner.

Category: Pseudomonas aeruginosa

Tags: aeruginosa, endotoxin, sepsis, septic shock