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Furthermore, even if we employ only the FEVj as a reliable indication of airflow obstruction and bronchial reactivity, we still find that 30 of the total 153 (20 percent) AIDS patients treated at this institution had abnormal airway function. Recognition of abnormal airway function had clinical importance in our patients. The symptoms of cough and […]

Tracheostomy tubes have been less well studied than endotracheal tubes. The pressure drop across tracheostomy tubes has beenmeasured by Yung and Snowden. Similarly, Cavo et al calculated tracheostomy tube resistances and compared them to upper airway resistances. At flows of 0.5 L/s, all but the largest tracheostomy tubes (8 or greater) showed airflow resistances greater […]

Cryopreservation of IVP bovine embryos derived from IVM oocytes has been successful at various developmental stages. In general, however, because IVP embryos are more sensitive to hypothermic conditions than in vivo-pro-duced embryos, they have lower viability following cryopreservation. Prolonged in vitro culture of oocytes and embryos results in disturbed membrane fluidity and permeability due to […]

Experiment 1 Table 1 summarizes the results of cryopreserving IVP embryos vitrified at the expanded blastocyst stage, where about 40% of the IVP blastocysts survived vitrification (Fig. 2a), although their average cell number was lower compared to that of the nonvitrified blastocysts (38.4 ± 5.1 vs. 61.9 ± 5.8, P < 0.05). Note that survival […]

Chemicals Unless otherwise indicated chemicals were obtained from Sigma Chemical Company (St. Louis, MO). In Vitro Maturation of Oocytes Ovaries were collected at a local abattoir and transported to the laboratory in PBS containing 75 ^g/ml potassium penicillin G, 50 ^g/ml streptomycin sulfate, and 0.1% (w/v) polyvinyl alcohol (PVA). Cumulus-oo-cyte complexes (COCs) were collected from […]

Cryopreservation technology applied to mammalian embryos of livestock such as cattle, goats, sheep, and other model animals has led to many successful developments since the 1980s. This, however, is not the case regarding cryopreservation of porcine embryos, which has faced many challenges over the years. It has only fairly recently become feasible to produce progeny […]