Another aliquot of the BAL fluid was analyzed for cellular content. Two to four hundred microliters of unconcentrated BAL fluid was spun onto glass slides using a cytocentrifuge (Cytospin II, Shandon Southern Instruments, Sewickley, PA). Slides were stained with a modified Wright-Giemsa stain (Diff-Quik, American Scientific Products, McGaw Park, IL). Cell differential counts were performed on 200 nucleated cells per slide using standard morphologic criteria established for our laboratory.
Chest roentgenograms were performed in all patients 24 h prior to bronchoscopy. The chest roentgenograms were divided into six possible groups: (1) cavitary (one or more cavities of greater than 5 mm in diameter); (2) infiltrate (infiltrate in two or fewer lobes of the lung without cavities seen); (3) nodule (one or more discrete nodules without associated infiltrate); (4) miliary (diffuse reticulonodular infiltrate involving more than two lobes); (5) effusion (pleural effusion without associated parenchymal disease except volume loss); and (6) normal (no abnormalities seen). buy yasmin online
In addition, the patients’ charts were reviewed to identify any possible underlying disease which may have led to increased risk for infection. Among the possible causes identified were AIDS, leukemia, lymphoma, solid organ malignancies, chronic corticosteroid administration and solid organ transplantation.